‚ÄčBonini

Marcelo G. Bonini, Ph.D.
Associate Professor
phone: (312) 355-5948
fax: (312) 996-4812
email: mbonini@uic.edu

 

 

Research Areas

  • Mitochondrial Redox Control of the Cellular Metabolism
  • S-Nitrosation inhibits Suppressor of Cytokine Signaling-1 (SOCS-1) allowing NF-kB activation in response to TLR4 activation 

Research Focus

1.  Mitochondrial Redox Control of the Cellular Metabolism:
This project is based on the hypothesis that mitochondria dynamically decide whether to utilize available oxygen for ATP production or H2O2 generation. H2O2 egressing from mitochondria initiates redox signaling, generation of oxidants is controlled by sophisticated mechanisms of electron transfer in part controlled by the enzyme manganese superoxide dismutase MnSOD. In cancer, MnSOD is constitutively unregulated and as shown in our studies the levels of MnSOD upregulation parallels tumor stage. By maintaining a steady flow of H2O2 originating in mitochondria MnSOD sustains AMP-activated kinase (AMPK) activity that produces the glycolytic shift that induces tumor cell energetic viability.

2.  S-Nitrosation inhibits Suppressor of Cytokine Signaling-1 (SOCS-1) allowing NF-kB activation in response to TLR4 activation:
This project is based on the observation that NOS1 activation in response to TLR4 activation is critical for initiation of NF-kB driven inflammation in macrophages. Our studies showed that NOS1 S-nitrosates, and thereby inhibits SOCS1. In its inhibited state SOCS1 cannot target the transcriptionally active NF-kB subunit p65 to proteasomal degradation allowing for the synthesis of pro inflammatory mediators. These studies indicate that the duration and intensity of signals originating from NOS1 define the course, locality and duration of inflammation.  

What I Do

My interests revolve around the central question of how oxidants modify the activity of enzymes implicated in the regulation of metabolic functions and gene transcription. Oxygen utilization is central to aerobic life and disturbances in how oxygen (and its metabolites) are handled broadly impact cellular signaling affecting health and promoting disease. Our laboratory focuses on identifying signaling networks susceptible to oxidative modification and then intervene in order to restore their proper function.

Biography

I was awarded my Ph.D. in Biochemistry and Molecular Biology in 2004 by the University of Sao Paulo, (Brazil). In 2004 I moved to laboratory of Dr. Ronald P. Mason, at the National Institute of Environmental Health Sciences for my postdoctoral training focused on the understanding of how redox driven reactions affect signaling events relevant for the maintenance of the cellular homeostatic balance and disease.  In 2009 I established an independent, multidisciplinary program focused on the identification of redox sensitive pathways involved in tumor progression and metastasis at the University of Illinois at Chicago (my current position).

Education

PhD, Biochemistry and Molecular Biology, 2004: University of Sao Paulo, Brazil

Fellowship

2004-2009:  Postdoctoral fellow, National Institute of Environmental Health Sciences

Research Expertise

Redox Biology, Metabolism, Mitochondria, Reactive Oxygen Species, Electron Paramagnetic Resonance

Awards and Honors

  • 2011        Ad. Hoc Reviewer Department of Defense Army Research Office (DOD/ARO)
  • 2012        Ad. Hoc Reviewer North Carolina Biotechnology Center – Biotechnology Research Grant Program
  • 2012        American Heart Association Regular Study Section Member (Molecular Signaling 1 & 2)
  • 2012        NIEHS/NIH study section member ZES1 LWJ-D (SF)
  • 2012        SFRBM – travel award/YIA to Kristine Ansenberger-Fricano
  • 2012        Constance Campbell memorial award from the Illinois symposium on reproductive sciences to Kristine Ansenberger-Fricano.
  • 2013 -      NIH Study Section Member, DKUS 90C (SIEE) Systemic Injury by Environmental Exposures
  • 2014        UIC/College of Medicine, Faculty of the year (Rising Star), Office of the Vice Chancellor for Faculty Affairs

Selected Recent Publications

Click to View Recent Publications

Keywords

Oxidative stress, mitochondria, ROS, metabolism, cancer.